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XB-ART-49181
Chembiochem 2014 Aug 18;1512:1710-20. doi: 10.1002/cbic.201402080.
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In vivo incorporation of non-canonical amino acids by using the chemical aminoacylation strategy: a broadly applicable mechanistic tool.

Dougherty DA , Van Arnam EB .


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We describe a strategy for incorporating non-canonical amino acids site-specifically into proteins expressed in living cells, involving organic synthesis to chemically aminoacylate a suppressor tRNA, protein expression in Xenopus oocytes, and monitoring protein function, primarily by electrophysiology. With this protocol, a very wide range of non-canonical amino acids can be employed, allowing both systematic structure-function studies and the incorporation of reactive functionalities. Here, we present an overview of the methodology and examples meant to illustrate the versatility and power of the method as a tool for investigating protein structure and function.

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Genes referenced: mt-tr trna

References [+] :
Ahern, A cation-pi interaction between extracellular TEA and an aromatic residue in potassium channels. 2006, Pubmed