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XB-ART-49454
Dev Dyn 2014 Dec 01;24312:1606-18. doi: 10.1002/dvdy.24193.
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Early stages of induction of anterior head ectodermal properties in Xenopus embryos are mediated by transcriptional cofactor ldb1.

Plautz CZ , Zirkle BE , Deshotel MJ , Grainger RM .


Abstract
BACKGROUND: Specific molecules involved in early inductive signaling from anterior neural tissue to the placodal ectoderm to establish a lens-forming bias, as well as their regulatory factors, remain largely unknown. In this study, we sought to identify and characterize these molecules. RESULTS: Using an expression cloning strategy to isolate genes with lens-inducing activity, we identified the transcriptional cofactor ldb1. This, together with evidence for its nuclear dependence, suggests its role as a regulatory factor, not a direct signaling molecule. We propose that ldb1 mediates induction of early lens genes in our functional assay by transcriptional activation of lens-inducing signals. Gain-of-function assays demonstrate that the inductive activity of the anterior neural plate on head ectodermal structures can be augmented by ldb1. Loss-of-function assays show that knockdown of ldb1 leads to decreased expression of early lens and retinal markers and subsequently to defects in eye development. CONCLUSIONS: The functional cloning, expression pattern, overexpression, and knockdown data show that an ldb1-regulated mechanism acts as an early signal for Xenopus lens induction.

PubMed ID: 25258326
PMC ID: PMC4237673
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ag1 dll1 dlx5 foxe3 ldb1 maf nrl rax
Morpholinos: ldb1 MO1


Article Images: [+] show captions
References [+] :
Agulnick, Interactions of the LIM-domain-binding factor Ldb1 with LIM homeodomain proteins. 1996, Pubmed, Xenbase