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Science 2014 Oct 24;3466208:477-81. doi: 10.1126/science.1253585.
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Polyubiquitylation drives replisome disassembly at the termination of DNA replication.

Moreno SP , Bailey R , Campion N , Herron S , Gambus A .

Resolution of replication forks during termination of DNA replication is essential for accurate duplication of eukaryotic genomes. Here we present evidence consistent with the idea that polyubiquitylation of a replisome component (Mcm7) leads to its disassembly at the converging terminating forks because of the action of the p97/VCP/Cdc48 protein remodeler. Using Xenopus laevis egg extract, we have shown that blocking polyubiquitylation results in the prolonged association of the active helicase with replicating chromatin. The Mcm7 subunit is the only component of the active helicase that we find polyubiquitylated during replication termination. The observed polyubiquitylation is followed by disassembly of the active helicase dependent on p97/VCP/Cdc48. Altogether, our data provide insight into the mechanism of replisome disassembly during eukaryotic DNA replication termination.

PubMed ID: 25342805
Article link: Science
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: eif4g2 mcm7 vcp
Antibodies: Recql4 Ab2

References [+] :
Bell, DNA Replication. Terminating the replisome. 2014, Pubmed