Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Sci Signal 2014 Mar 04;7315:ra22. doi: 10.1126/scisignal.2005025.
Show Gene links Show Anatomy links

KCNQ1, KCNE2, and Na+-coupled solute transporters form reciprocally regulating complexes that affect neuronal excitability.

Abbott GW , Tai KK , Neverisky DL , Hansler A , Hu Z , Roepke TK , Lerner DJ , Chen Q , Liu L , Zupan B , Toth M , Haynes R , Huang X , Demirbas D , Buccafusca R , Gross SS , Kanda VA , Berry GT .

Na(+)-coupled solute transport is crucial for the uptake of nutrients and metabolic precursors, such as myo-inositol, an important osmolyte and precursor for various cell signaling molecules. We found that various solute transporters and potassium channel subunits formed complexes and reciprocally regulated each other in vitro and in vivo. Global metabolite profiling revealed that mice lacking KCNE2, a K(+) channel β subunit, showed a reduction in myo-inositol concentration in cerebrospinal fluid (CSF) but not in serum. Increased behavioral responsiveness to stress and seizure susceptibility in Kcne2(-/-) mice were alleviated by injections of myo-inositol. Suspecting a defect in myo-inositol transport, we found that KCNE2 and KCNQ1, a voltage-gated potassium channel α subunit, colocalized and coimmunoprecipitated with SMIT1, a Na(+)-coupled myo-inositol transporter, in the choroid plexus epithelium. Heterologous coexpression demonstrated that myo-inositol transport by SMIT1 was augmented by coexpression of KCNQ1 but was inhibited by coexpression of both KCNQ1 and KCNE2, which form a constitutively active, heteromeric K(+) channel. SMIT1 and the related transporter SMIT2 were also inhibited by a constitutively active mutant form of KCNQ1. The activities of KCNQ1 and KCNQ1-KCNE2 were augmented by SMIT1 and the glucose transporter SGLT1 but were suppressed by SMIT2. Channel-transporter signaling complexes may be a widespread mechanism to facilitate solute transport and electrochemical crosstalk.

PubMed ID: 24595108
PMC ID: PMC4063528
Article link: Sci Signal
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: kcne2 kcnq1 slc5a1.2 slc5a3

References [+] :
Abbott, MiRP1 forms IKr potassium channels with HERG and is associated with cardiac arrhythmia. 1999, Pubmed, Xenbase