Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Development 2015 Feb 01;1423:486-96. doi: 10.1242/dev.108266.
Show Gene links Show Anatomy links

Regulation of ECM degradation and axon guidance by growth cone invadosomes.

Santiago-Medina M , Gregus KA , Nichol RH , O'Toole SM , Gomez TM .

Invadopodia and podosomes, collectively referred to as invadosomes, are F-actin-rich basal protrusions of cells that provide sites of attachment to and degradation of the extracellular matrix. Invadosomes promote the invasion of cells, ranging from metastatic cancer cells to immune cells, into tissue. Here, we show that neuronal growth cones form protrusions that share molecular, structural and functional characteristics of invadosomes. Growth cones from all neuron types and species examined, including a variety of human neurons, form invadosomes both in vitro and in vivo. Growth cone invadosomes contain dynamic F-actin and several actin regulatory proteins, as well as Tks5 and matrix metalloproteinases, which locally degrade the matrix. When viewed using three-dimensional super-resolution microscopy, F-actin foci often extended together with microtubules within orthogonal protrusions emanating from the growth cone central domain. Finally, inhibiting the function of Tks5 both reduced matrix degradation in vitro and disrupted motoneuron axons from exiting the spinal cord and extending into the periphery. Taken together, our results suggest that growth cones use invadosomes to target protease activity during axon guidance through tissues.

PubMed ID: 25564649
PMC ID: PMC4302990
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actb actl6a actn1 actr3 adam17 b3gat1l cttn enah fn1 itgb1 mmp14 mmp2 ncam1 pmch ptk2 pxn sh3pxd2a src syt2 tuba4b tubb2b tubb3 was wasl
Antibodies: B3gat1 Ab3 Cttn Ab1 Enah Ab1 GFP Ab18 Itgb1 Ab1 Mmp14 Ab1 Ptk2 Ab1 Pxn Ab2 Sh3pxd2a Ab1 Src Ab2 Sv2a Ab1 Syt2 Ab1 Tuba4b Ab5 Tubulin I+II Ab1 Wasl Ab1 actr3 Ab1

Article Images: [+] show captions
References [+] :
Abram, The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells. 2003, Pubmed