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Genesis 2015 May 01;535:299-307. doi: 10.1002/dvg.22851.
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Contractile activity is required for Z-disc sarcomere maturation in vivo.

Geach TJ , Hirst EM , Zimmerman LB .

Sarcomere structure underpins structural integrity, signaling, and force transmission in the muscle. In embryos of the frog Xenopus tropicalis, muscle contraction begins even while sarcomerogenesis is ongoing. To determine whether contractile activity plays a role in sarcomere formation in vivo, chemical tools were used to block acto-myosin contraction in embryos of the frog X. tropicalis, and Z-disc assembly was characterized in the paralyzed dicky ticker mutant. Confocal and ultrastructure analysis of paralyzed embryos showed delayed Z-disc formation and defects in thick filament organization. These results suggest a previously undescribed role for contractility in sarcomere maturation in vivo.

PubMed ID: 25845369
PMC ID: PMC4676352
Article link: Genesis
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: actn1 actn2 actn3 elob myh6 tcea1 unc45b
Antibodies: Actn2 Ab2 Myh6 Ab1

Article Images: [+] show captions
References [+] :
Abu-Daya, Absence of heartbeat in the Xenopus tropicalis mutation muzak is caused by a nonsense mutation in cardiac myosin myh6. 2009, Pubmed, Xenbase