Kelwick R , Desanlis I , Wheeler GN , Edwards DR .

Fig 1. The ADAMTS (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) family. The basic domain organization of the 19 ADAMTS family members and their major functional groups. Structurally the ADAMTS members are broadly organized into a proteinase domain and an ancillary domain. The proteinase domain comprises the signal, pro, metalloproteinase and disintegrin-like domains. The greatest variability between ADAMTS members is found in the ancillary domain, which is composed of one or more thrombospondin type 1 sequence repeats (TSRs), a cysteine-rich domain and a spacer domain. Some family members also have one or more specialist domains as part of their ancillary domain, as listed in the key on the right. The diagram is drawn to scale

Fig. 2. Evolution of the ADAMTS family. a A schematic representation of the relationships of the eight vertebrate ADAMTS clades and the probable events (gene duplications and a retrotransposition) that have contributed to the expansion of the family. The figure is not to scale in terms of evolutionary distance. COMP, cartilage oligomeric protein; vWFCP, von-Willebrand-factor-cleaving protease. b Phylogenetic tree of the ADAMTS genes inferred by the maximum likelihood method based on the JTT matrix-based model [144]. The bootstrap consensus tree inferred from 1,000 replicates was taken to represent the evolutionary history of the taxa analyzed [145]. Branches corresponding to partitions reproduced in less than 50 % bootstrap replicates were collapsed. Initial tree(s) for the heuristic search were obtained by applying the neighbor-joining method to a matrix of pairwise distances estimated using a JTT model. The analysis involved 70 amino acid sequences. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA6 [146]. The vertebrate ADAMTS genes are indicated by a number followed by a single letter code indicating the species: for example, 1 t represents 1_t ADAMTS1 from Xenopus tropicalis; 15 h is 15_h ADAMTS15 from Homo sapiens; 8 m is ADAMTS8 from Mus musculus. For Drosophila melanogaster (dro), Caenorhabditis elegans (cel) and Ciona intestinalis (cio), the annotation is species followed by the gene number; for example, cio 6 is cio_6 ADAMTS6 from Ciona

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