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Sci Rep 2015 Dec 21;5:18519. doi: 10.1038/srep18519.
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Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis.

Tanizaki Y , Ichisugi M , Obuchi-Shimoji M , Ishida-Iwata T , Tahara-Mogi A , Meguro-Ishikawa M , Kato T .

The development of mammalian megakaryocytes (MKs) and platelets, which are thought to be absent in non-mammals, is primarily regulated by the thrombopoietin (TPO)/Mpl system. Although non-mammals possess nucleated thrombocytes instead of platelets, the features of nucleated thrombocyte progenitors remain to be clarified. Here, we provide the general features of TPO using Xenopus laevis TPO (xlTPO). Hepatic and splenic cells were cultured in liquid suspension with recombinant xlTPO. These cells differentiated into large, round, polyploid CD41-expressing cells and were classified as X. laevis MKs, comparable to mammalian MKs. The subsequent culture of MKs after removal of xlTPO produced mature, spindle-shaped thrombocytes that were activated by thrombin, thereby altering their morphology. XlTPO induced MKs in cultured hepatic cells for at least three weeks; however, this was not observed in splenic cells; this result demonstrates the origin of early haematopoietic progenitors in the liver rather than the spleen. Additionally, xlTPO enhanced viability of peripheral thrombocytes, indicating the xlTPO-Mpl pathway stimulates anti-apoptotic in peripheral thrombocytes. The development of thrombocytes from MKs via the TPO-Mpl system in X. laevis plays a crucial role in their development from MKs, comparable to mammalian thrombopoiesis. Thus, our results offer insight into the cellular evolution of platelets/MKs in vertebrates. (200/200).

PubMed ID: 26687619
PMC ID: PMC4685256
Article link: Sci Rep

Species referenced: Xenopus laevis
Genes referenced: ache chrd epor fli1 itga2b.1 jak2 mpl mpo stat5b tbl1x tbx2 thpo tpo
Antibodies: Thrombocyte Ab1

Article Images: [+] show captions
References [+] :
Aizawa, Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form. 2005, Pubmed, Xenbase