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J Cell Biol 2003 May 26;1614:769-77. doi: 10.1083/jcb.200211094.
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Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos.

Sheldahl LC , Slusarski DC , Pandur P , Miller JR , Kühl M , Moon RT .

Wnt ligands and Frizzled (Fz) receptors have been shown to activate multiple intracellular signaling pathways. Activation of the Wnt-beta-catenin pathway has been described in greatest detail, but it has been reported that Wnts and Fzs also activate vertebrate planar cell polarity (PCP) and Wnt-Ca2+ pathways. Although the intracellular protein Dishevelled (Dsh) plays a dual role in both the Wnt-beta-catenin and the PCP pathways, its potential involvement in the Wnt-Ca2+ pathway has not been investigated. Here we show that a Dsh deletion construct, XDshDeltaDIX, which is sufficient for activation of the PCP pathway, is also sufficient for activation of three effectors of the Wnt-Ca2+ pathway: Ca2+ flux, PKC, and calcium/calmodulin-dependent protein kinase II (CamKII). Furthermore, we find that interfering with endogenous Dsh function reduces the activation of PKC by Xfz7 and interferes with normal heart development. These data suggest that the Wnt-Ca2+ pathway utilizes Dsh, thereby implicating Dsh as a component of all reported Fz signaling pathways.

PubMed ID: 12771126
PMC ID: PMC2199364
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: camk2g dvl1 dvl2 fzd7 tbx2 tnni3 wnt5a
Morpholinos: dvl2 MO1

Article Images: [+] show captions
References [+] :
Adler, Frizzled signaling and cell-cell interactions in planar polarity. 2001, Pubmed