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Dev Biol 2016 Aug 15;4162:361-72. doi: 10.1016/j.ydbio.2016.06.012.
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The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification.

Hatch VL , Marin-Barba M , Moxon S , Ford CT , Ward NJ , Tomlinson ML , Desanlis I , Hendry AE , Hontelez S , van Kruijsbergen I , Veenstra GJ , Münsterberg AE , Wheeler GN .

Regulation of gene expression at the level of transcriptional elongation has been shown to be important in stem cells and tumour cells, but its role in the whole animal is only now being fully explored. Neural crest cells (NCCs) are a multipotent population of cells that migrate during early development from the dorsal neural tube throughout the embryo where they differentiate into a variety of cell types including pigment cells, cranio-facial skeleton and sensory neurons. Specification of NCCs is both spatially and temporally regulated during embryonic development. Here we show that components of the transcriptional elongation regulatory machinery, CDK9 and CYCLINT1 of the P-TEFb complex, are required to regulate neural crest specification. In particular, we show that expression of the proto-oncogene c-Myc and c-Myc responsive genes are affected. Our data suggest that P-TEFb is crucial to drive expression of c-Myc, which acts as a 'gate-keeper' for the correct temporal and spatial development of the neural crest.

PubMed ID: 27343897
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ccnt1 cdk9 egr2 elavl4 foxd3 hsp90aa1.1 hsph1 isl1 myc ncam1 neurod1 neurog2 nog otx2 pax3 polr2a rpl13 runx1 snai1 snai2 sox1 sox10 sox2 sox8 sox9 tbx2 tubb2b wnt1 zic1 zic3
GO keywords: transcription elongation factor complex [+]
Antibodies: polr2a Ab8
Morpholinos: ccnt1 MO1 cdk9 MO1 cdk9 MO2

GEO Series: GSE82153: NCBI

Article Images: [+] show captions