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Heliyon 2016 Sep 24;29:e00166. doi: 10.1016/j.heliyon.2016.e00166.
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Gamma-tubulin coordinates nuclear envelope assembly around chromatin.

Rosselló CA , Lindström L , Glindre J , Eklund G , Alvarado-Kristensson M .

The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.

PubMed ID: 27699285
PMC ID: PMC5037270
Article link: Heliyon

Species referenced: Xenopus laevis
Genes referenced: cdk5rap3 e2f1 gtf2i gtf2ird1 h2bc21 lamb1 lamb3 rad51 tubg1 tubgcp2 tubgcp3
GO keywords: nucleus [+]

Article Images: [+] show captions
References [+] :
Alvarado-Kristensson, p38 Mitogen-activated protein kinase and phosphatidylinositol 3-kinase activities have opposite effects on human neutrophil apoptosis. 2002, Pubmed