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Dev Dyn 2017 Feb 01;2462:100-115. doi: 10.1002/dvdy.24470.
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Role of JNK during buccopharyngeal membrane perforation, the last step of embryonic mouth formation.

Houssin NS , Bharathan NK , Turner SD , Dickinson AJ .

BACKGROUND: The buccopharyngeal membrane is a thin layer of cells covering the embryonic mouth. The perforation of this structure creates an opening connecting the external and the digestive tube which is essential for oral cavity formation. In humans, persistence of the buccopharyngeal membrane can lead to orofacial defects such as choanal atresia, oral synechiaes, and cleft palate. Little is known about the causes of a persistent buccopharyngeal membrane and, importantly, how this structure ruptures. RESULTS: We have determined, using antisense and pharmacological approaches, that Xenopus embryos deficient c-Jun N-terminal kinase (JNK) signaling have a persistent buccopharyngeal membrane. JNK deficient embryos have decreased cell division and increased cellular stress and apoptosis. However, altering these processes independently of JNK did not affect buccopharyngeal membrane perforation. JNK deficient embryos also have increased intercellular adhesion and defects in e-cadherin localization. Conversely, embryos with overactive JNK have epidermal fragility, increased E-cadherin internalization, and increased membrane localized clathrin. In the buccopharyngeal membrane, clathrin is colocalized with active JNK. Furthermore, inhibition of endocytosis results in a persistent buccopharyngeal membrane, mimicking the JNK deficient phenotype. CONCLUSIONS: The results of this study suggest that JNK has a role in the disassembly adherens junctions by means of endocytosis that is required during buccopharyngeal membrane perforation. Developmental Dynamics 246:100-115, 2017. © 2016 Wiley Periodicals, Inc.

PubMed ID: 28032936
PMC ID: PMC5261731
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: casp3.2 cdh1 cltc gspt1 gstp1 h3-3a jun mapk8
GO keywords: cell-cell adherens junction
Antibodies: Casp3 Ab1 Cdh1 Ab1 H3f3a Ab9 cltc Ab2 mapk8 Ab3
Morpholinos: mapk8 MO1 mapk8 MO2

Article Images: [+] show captions
References [+] :
Bennett, SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. 2001, Pubmed