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Sci Rep 2015 Jan 12;5:18581. doi: 10.1038/srep18581.
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TRPV4 associates environmental temperature and sex determination in the American alligator.

Yatsu R , Miyagawa S , Kohno S , Saito S , Lowers RH , Ogino Y , Fukuta N , Katsu Y , Ohta Y , Tominaga M , Guillette LJ , Iguchi T .

Temperature-dependent sex determination (TSD), commonly found among reptiles, is a sex determination mode in which the incubation temperature during a critical temperature sensitive period (TSP) determines sexual fate of the individual rather than the individual's genotypic background. In the American alligator (Alligator mississippiensis), eggs incubated during the TSP at 33 °C (male producing temperature: MPT) yields male offspring, whereas incubation temperatures below 30 °C (female producing temperature: FPT) lead to female offspring. However, many of the details of the underlying molecular mechanism remains elusive, and the molecular link between environmental temperature and sex determination pathway is yet to be elucidated. Here we show the alligator TRPV4 ortholog (AmTRPV4) to be activated at temperatures proximate to the TSD-related temperature in alligators, and using pharmacological exposure, we show that AmTRPV4 channel activity affects gene expression patterns associated with male differentiation. This is the first experimental demonstration of a link between a well-described thermo-sensory mechanism, TRPV4 channel, and its potential role in regulation of TSD in vertebrates, shedding unique new light on the elusive TSD molecular mechanism.

PubMed ID: 26677944
PMC ID: PMC4683465
Article link: Sci Rep

Species referenced: Xenopus
Genes referenced: amh cyp19a1 sox9 trpv4

Article Images: [+] show captions
References [+] :
Argentaro, A SOX9 defect of calmodulin-dependent nuclear import in campomelic dysplasia/autosomal sex reversal. 2003, Pubmed