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PLoS Genet 2016 Jul 28;127:e1006221. doi: 10.1371/journal.pgen.1006221.
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FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC.

Charles Richard JL , Shukla MS , Menoni H , Ouararhni K , Lone IN , Roulland Y , Papin C , Ben Simon E , Kundu T , Hamiche A , Angelov D , Dimitrov S .

FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.

PubMed ID: 27467129
PMC ID: PMC4965029
Article link: PLoS Genet

Species referenced: Xenopus laevis
Genes referenced: ctrl ogg1 ssrp1

Article Images: [+] show captions
References [+] :
Angelov, The histone octamer is invisible when NF-kappaB binds to the nucleosome. 2004, Pubmed, Xenbase