Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Biol 2017 May 01;4251:33-43. doi: 10.1016/j.ydbio.2017.03.015.
Show Gene links Show Anatomy links

sall1 and sall4 repress pou5f3 family expression to allow neural patterning, differentiation, and morphogenesis in Xenopus laevis.

Exner CRT , Kim AY , Mardjuki SM , Harland RM .

The embryonic precursor of the vertebrate central nervous system, the neural plate, is patterned along the anterior-posterior axis and shaped by morphogenetic movements early in development. We previously identified the genes sall1 and sall4, known regulators of pluripotency in other contexts, as transcriptional targets of developmental signaling pathways that regulate neural development. Here, we demonstrate that these two genes are required for induction of posterior neural fates, the cell shape changes that contribute to neural tube closure, and later neurogenesis. Upon sall1 or sall4 knockdown, defects are associated with the failure of the neural plate to differentiate. Consistent with this, sall-deficient neural tissue exhibits an aberrant upregulation of pou5f3 family genes, the Xenopus homologs of the mammalian stem cell maintenance factor Pou5f1 (Oct4). Furthermore, overexpression of pou5f3 genes in Xenopus causes defects in neural patterning, morphogenesis, and differentiation that phenocopy those observed in sall1 and sall4 morphants. In all, this work shows that both sall1 and sall4 act to repress pou5f3 family gene expression in the neural plate, thereby allowing vertebrate neural development to proceed.

PubMed ID: 28322736
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actb egr2 gata2 hoxb9 hoxd10 krt12.4 not otx2 pax3 pax6 pou5f3 pou5f3.2 pou5f3.3 rab11a sall1 sall4 shroom3 snai2 sox2 tuba4b tubb2b
Antibodies: FITC-phalloidin Lectin Tuba4b Ab2

Article Images: [+] show captions