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J Comp Neurol 2003 Jun 23;4612:262-75. doi: 10.1002/cne.10695.
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Increased expression of multiple neurofilament mRNAs during regeneration of vertebrate central nervous system axons.

Gervasi C , Thyagarajan A , Szaro BG .

Characteristic changes in the expression of neuronal intermediate filaments (nIFs), an abundant cytoskeletal component of vertebrate axons, accompany successful axon regeneration. In mammalian regenerating PNS, expression of nIFs that are characteristic of mature neurons becomes suppressed throughout regeneration, whereas that of peripherin, which is abundant in developing axons, increases. Comparable changes are absent from mammalian injured CNS; but in goldfish and lamprey CNS, expression of several nIFs increases during axon regrowth. To obtain a broader view of the nIF response of successfully regenerating vertebrate CNS, in situ hybridization and video densitometry were used to track multiple nIF mRNAs during optic axon regeneration in Xenopus laevis. As in other successfully regenerating systems, peripherin expression increased rapidly after injury and expression of those nIFs characteristic of mature retinal ganglion cells decreased. Unlike the decrease in nIF mRNAs of regenerating PNS, that of Xenopus retinal ganglion cells was transient, with most nIF mRNAs increasing above normal during axon regrowth. At the peak of regeneration, increases in each nIF mRNA resulted in a doubling of the total amount of nIF mRNA, as well as a shift in the relative proportions contributed by each nIF. The relative proportions of peripherin and NF-M increased above normal, whereas proportions of xefiltin and NF-L decreased and that of XNIF remained the same. The increases in peripherin and NF-M mRNAs were accompanied by increases in protein. These results are consistent with the hypothesis that successful axon regeneration involves changes in nIF subunit composition conducive to growth and argue that a successful injury response differs between CNS and PNS.

PubMed ID: 12724842
Article link: J Comp Neurol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ctdspl ina neff1 nefl nefm nfs1 prph
Antibodies: Nefm Ab1 Nefm Ab4 Prph Ab1 Prph Ab2 Prph Ab3 phospho-Nefm Ab3

Article Images: [+] show captions