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Sci Rep 2017 Nov 15;71:15647. doi: 10.1038/s41598-017-15936-y.
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Phosphorylation Dynamics Dominate the Regulated Proteome during Early Xenopus Development.

Peuchen EH , Cox OF , Sun L , Hebert AS , Coon JJ , Champion MM , Dovichi NJ , Huber PW .

The earliest stages of animal development are largely controlled by changes in protein phosphorylation mediated by signaling pathways and cyclin-dependent kinases. In order to decipher these complex networks and to discover new aspects of regulation by this post-translational modification, we undertook an analysis of the X. laevis phosphoproteome at seven developmental stages beginning with stage VI oocytes and ending with two-cell embryos. Concurrent measurement of the proteome and phosphoproteome enabled measurement of phosphosite occupancy as a function of developmental stage. We observed little change in protein expression levels during this period. We detected the expected phosphorylation of MAP kinases, translational regulatory proteins, and subunits of APC/C that validate the accuracy of our measurements. We find that more than half the identified proteins possess multiple sites of phosphorylation that are often clustered, where kinases work together in a hierarchical manner to create stretches of phosphorylated residues, which may be a means to amplify signals or stabilize a particular protein conformation. Conversely, other proteins have opposing sites of phosphorylation that seemingly reflect distinct changes in activity during this developmental timeline.

PubMed ID: 29142207
PMC ID: PMC5688136
Article link: Sci Rep
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: anapc1 arrb1 arrb2 atm aurka bub1 ccne1 ccne2 cdc20 cdc25a cdc6 cdk1 cdk2 clasp1 cpeb1 csnk1a1 csnk2b dlgap5 eif3a eif4e eif4g1 eif4g2 elavl1 fmr1 gsk3a gsk3b gys1 lmnb1 lmnb3 map2k1 map4 mapk12 marcks mcm2 melk mki67 mos msi1 myt1 nol8 nolc1 pcm1 pin1 pkmyt1 plk1 prkcd rps6ka1 rps6ka3 sympk upk1b wee1 zp2 zp3 zp4 zpax
GO keywords: oocyte maturation [+]

Article Images: [+] show captions
References [+] :
Amanchy, A curated compendium of phosphorylation motifs. 2007, Pubmed