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Cell Rep 2018 Feb 27;229:2265-2278. doi: 10.1016/j.celrep.2018.02.015.
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A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis.

Christov CP , Dingwell KS , Skehel M , Wilkes HS , Sale JE , Smith JC , Krude T .

DNA replication in the embryo of Xenopus laevis changes dramatically at the mid-blastula transition (MBT), with Y RNA-independent random initiation switching to Y RNA-dependent initiation at specific origins. Here, we identify xNuRD, an MTA2-containing assemblage of the nucleosome remodeling and histone deacetylation complex NuRD, as an essential factor in pre-MBT Xenopus embryos that overcomes a functional requirement for Y RNAs during DNA replication. Human NuRD complexes have a different subunit composition than xNuRD and do not support Y RNA-independent initiation of DNA replication. Blocking or immunodepletion of xNuRD inhibits DNA replication initiation in isolated nuclei in vitro and causes inhibition of DNA synthesis, developmental delay, and embryonic lethality in early embryos. xNuRD activity declines after the MBT, coinciding with dissociation of the complex and emergence of Y RNA-dependent initiation. Our data thus reveal an essential role for a NuRD complex as a DNA replication factor during early Xenopus development.

PubMed ID: 29490265
PMC ID: PMC5848848
Article link: Cell Rep
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actg1 cat.1 cct4 chd4 copa copb2 ddx4 eif3a eif3l eprs1 gatad2a hdac1 hdac3 kars1 kpna1 mbd3 mcm7 mta1 mta2 npm1 psmc3 qars1 rars1 rbbp4 rbbp7 rny3 rps11 rps2 rps9
GO keywords: DNA replication [+]
Antibodies: GFP Ab18 actg1 Ab1 chd4 Ab1 ddx4 Ab 2 gatad2a Ab1 hdac1 Ab1 mbd3 Ab1 mta1 Ab1 mta2 Ab1 rbbp4 Ab1 rbbp7 Ab1
Morpholinos: rny3 MO1

Article Images: [+] show captions
References [+] :
Allen, The NuRD architecture. 2013, Pubmed