Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Science 2019 May 17;3646441:653-658. doi: 10.1126/science.aav9996.
Show Gene links Show Anatomy links

Identification of a regeneration-organizing cell in the Xenopus tail.

Aztekin C , Hiscock TW , Marioni JC , Gurdon JB , Simons BD , Jullien J .

Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration.

PubMed ID: 31097661
PMC ID: PMC6986927
Article link: Science
Grant support: [+]

Species referenced: Xenopus laevis
GO keywords: regeneration [+]

References [+] :
Aibar, SCENIC: single-cell regulatory network inference and clustering. 2017, Pubmed