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Development 2019 Aug 09;14615:. doi: 10.1242/dev.179580.
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Integration of Wnt and FGF signaling in the Xenopus gastrula at TCF and Ets binding sites shows the importance of short-range repression by TCF in patterning the marginal zone.

Kjolby RAS , Truchado-Garcia M , Iruvanti S , Harland RM .

During Xenopus gastrulation, Wnt and FGF signaling pathways cooperate to induce posterior structures. Wnt target expression around the blastopore falls into two main categories: a horseshoe shape with a dorsal gap, as in Wnt8 expression; or a ring, as in FGF8 expression. Using ChIP-seq, we show, surprisingly, that the FGF signaling mediator Ets2 binds near all Wnt target genes. However, β-catenin preferentially binds at the promoters of genes with horseshoe patterns, but further from the promoters of genes with ring patterns. Manipulation of FGF or Wnt signaling demonstrated that 'ring' genes are responsive to FGF signaling at the dorsal midline, whereas 'horseshoe' genes are predominantly regulated by Wnt signaling. We suggest that, in the absence of active β-catenin at the dorsal midline, the DNA-binding protein TCF binds and actively represses gene activity only when close to the promoter. In contrast, genes without functional TCF sites at the promoter may be predominantly regulated by Ets at the dorsal midline and are expressed in a ring. These results suggest recruitment of only short-range repressors to potential Wnt targets in the Xenopus gastrula.

PubMed ID: 31285353
PMC ID: PMC6703714
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: axin2l cdx1 cdx2 chrd crebbp ctnnb1 dkk1 eef1a1 eef1a2 ep300 ets1 ets2 fgf8 gsc ngfr pnhd tbxt tcf3 tcf7l1 wnt8a
GO keywords: gastrulation [+]

GEO Series: GSE112249: NCBI

Article Images: [+] show captions
References [+] :
Barolo, hairy mediates dominant repression in the Drosophila embryo. 1997, Pubmed