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Nat Commun 2020 Jun 19;111:3060. doi: 10.1038/s41467-020-16823-3.
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Distinct pseudokinase domain conformations underlie divergent activation mechanisms among vertebrate MLKL orthologues.

Davies KA , Fitzgibbon C , Young SN , Garnish SE , Yeung W , Coursier D , Birkinshaw RW , Sandow JJ , Lehmann WIL , Liang LY , Lucet IS , Chalmers JD , Patrick WM , Kannan N , Petrie EJ , Czabotar PE , Murphy JM .

The MLKL pseudokinase is the terminal effector in the necroptosis cell death pathway. Phosphorylation by its upstream regulator, RIPK3, triggers MLKL's conversion from a dormant cytoplasmic protein into oligomers that translocate to, and permeabilize, the plasma membrane to kill cells. The precise mechanisms underlying these processes are incompletely understood, and were proposed to differ between mouse and human cells. Here, we examine the divergence of activation mechanisms among nine vertebrate MLKL orthologues, revealing remarkable specificity of mouse and human RIPK3 for MLKL orthologues. Pig MLKL can restore necroptotic signaling in human cells; while horse and pig, but not rat, MLKL can reconstitute the mouse pathway. This selectivity can be rationalized from the distinct conformations observed in the crystal structures of horse and rat MLKL pseudokinase domains. These studies identify important differences in necroptotic signaling between species, and suggest that, more broadly, divergent regulatory mechanisms may exist among orthologous pseudoenzymes.

PubMed ID: 32561735
PMC ID: PMC7305131
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus
Genes referenced: diablo mapk1 mlkl ripk3

Article Images: [+] show captions
References [+] :
Adams, PHENIX: a comprehensive Python-based system for macromolecular structure solution. 2010, Pubmed