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Cold Spring Harb Protoc 2021 Apr 01;20214:. doi: 10.1101/pdb.prot104463.
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Live Imaging of Cytoskeletal Dynamics in Embryonic Xenopus laevis Growth Cones and Neural Crest Cells.

The cytoskeleton is a dynamic, fundamental network that not only provides mechanical strength to maintain a cell's shape but also controls critical events like cell division, polarity, and movement. Thus, how the cytoskeleton is organized and dynamically regulated is critical to our understanding of countless processes. Live imaging of fluorophore-tagged cytoskeletal proteins allows us to monitor the dynamic nature of cytoskeleton components in embryonic cells. Here, we describe a protocol to monitor and analyze cytoskeletal dynamics in primary embryonic neuronal growth cones and neural crest cells obtained from Xenopus laevis embryos.

PubMed ID: 33272974
PMC ID: PMC8026486
Article link: Cold Spring Harb Protoc
Grant support: [+]

Species referenced: Xenopus laevis

References [+] :
Applegate, plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics. 2012, Pubmed