Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-58672
Development 2021 Dec 01;14823:. doi: 10.1242/dev.200001.
Show Gene links Show Anatomy links

The mechanosensitive channel Piezo1 cooperates with semaphorins to control neural crest migration.

Canales Coutiño B , Mayor R .


Abstract
Cells are permanently exposed to a multitude of different kinds of signals: however, how cells respond to simultaneous extracellular signals within a complex in vivo environment is poorly understood. Here, we studied the role of the mechanosensitive ion channel Piezo1 on the migration of the neural crest, a multipotent embryonic cell population. We identify that Piezo1 is required for the migration of Xenopus cephalic neural crest. We show that loss of Piezo1 promotes focal adhesion turnover and cytoskeletal dynamics by controlling Rac1 activity, leading to increased speed of migration. Moreover, overactivation of Rac1, due to Piezo1 inhibition, counteracts cell migration inhibitory signals by Semaphorin 3A and Semaphorin 3F, generating aberrant neural crest invasion in vivo. Thus, we find that, for directional migration in vivo, neural crest cells require a tight regulation of Rac1, by semaphorins and Piezo1. We reveal here that a balance between a myriad of signals through Rac1 dictates cell migration in vivo, a mechanism that is likely to be conserved in other cell migration processes.

PubMed ID: 34822717
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: fas fn1 mrc1 piezo1 ptk2 pxn rac1 sema3a sema3f twist1
GO keywords: neural crest cell migration [+]
Antibodies: Piezo Ab1 Pxn Ab2 Vcl Ab1
Morpholinos: piezo1 MO1


Article Images: [+] show captions
References [+] :
Bae, The mechanosensitive ion channel Piezo1 is inhibited by the peptide GsMTx4. 2011, Pubmed