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Cell Calcium 2022 Mar 01;102:102540. doi: 10.1016/j.ceca.2022.102540.
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Injury-induced Erk1/2 signaling tissue-specifically interacts with Ca2+ activity and is necessary for regeneration of spinal cord and skeletal muscle.

Levin JB , Borodinsky LN .

The transition of stem cells from quiescence to proliferation enables tissues to self-repair. The signaling mechanisms driving these stem-cell-status decisions are still unclear. Ca2+ and the extracellular signal-regulated kinase (Erk1/2) are two signaling pathways that have the potential to coordinate multiple signals to promote a specific cellular response. They both play important roles during nervous system development but their roles during spinal cord and muscle regeneration are not fully deciphered. Here we show in Xenopus laevis larvae that both Ca2+ and Erk1/2 signaling pathways are activated after tail amputation. In response to injury, we find that Erk1/2 signaling is activated in neural and muscle stem cells and is necessary for spinal cord and skeletal muscle regeneration. Finally, we show in vivo that Erk1/2 activity is necessary for an injury-induced increase in intracellular store-dependent Ca2+ dynamics in skeletal muscle-associated tissues but that in spinal cord, injury increases Ca2+ influx-dependent Ca2+ activity independent of Erk1/2 signaling. This study suggests that precise temporal and tissue-specific activation of Ca2+ and Erk1/2 pathways is essential for regulating spinal cord and muscle regeneration.

PubMed ID: 35074688
PMC ID: PMC9542431
Article link: Cell Calcium
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: en1 map2k1 mapk1 pax7 pnma2 ptpn11 sox2
GO keywords: axon regeneration [+]
Antibodies: H3f3a Ab9 Mapk1/3 Ab20 Neuronal Ab5 Notochord Ab2 Pax7 Ab3 Somite Ab3 Sox2 Ab6
Morpholinos: map2k1 MO3

Article Images: [+] show captions
References [+] :
Allen, Hepatocyte growth factor activates quiescent skeletal muscle satellite cells in vitro. 1995, Pubmed