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Dev Biol 2022 Aug 01;488:81-90. doi: 10.1016/j.ydbio.2022.05.009.
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Xenopus Dusp6 modulates FGF signaling to precisely pattern pre-placodal ectoderm.

Tsukano K , Yamamoto T , Watanabe T , Michiue T .

Pre-placodal ectoderm (PPE), a horseshoe-shaped narrow region formed during early vertebrate development, gives rise to multiple types of sensory organs and ganglia. For PPE induction, a certain level of FGF signal activation is required. However, it is difficult to reproducibly induce the narrow region with variations in gene expression, including FGF, among individuals. An intracellular regulatory factor of FGF signaling, Dusp6, is expressed by FGF signal activation and inactivates a downstream regulator, ERK1/2, in adult tissues; however, its role in early development is not well known. Here, we reveal that Dusp6 is expressed in an FGF-dependent manner in Xenopus PPE. Gain- and loss-of-function experiments showed that Dusp6 is required for expression of a PPE gene, Six1, and patterning of adjacent regions, neural plate, and neural crest. To reveal the importance of Dusp6 in variable FGF production, we performed Dusp6 knockdown with FGF-bead implantation, which resulted in varying Six1 expression patterns. Taken together, these results suggest that Dusp6 is required for PPE formation and that it contributes to the robust patterning of PPE by mediating FGF signaling.

PubMed ID: 35598626
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: dusp6 eef1a1 egr2 eno1 eya1 fgf8 foxd3 krt12.4 krt12.5 mapk1 myod1 npb otx2 pax3 six1 sox3 zic1
GO keywords: ectoderm formation [+]
Antibodies: GFP Ab26 Mapk1 Ab10 Mapk1 Ab14 Tuba1b Ab1 Tubb2b Ab7

Article Images: [+] show captions