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Int J Mol Sci 2022 Aug 17;2316:. doi: 10.3390/ijms23169250.
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Insulin Receptor-Related Receptor Regulates the Rate of Early Development in Xenopus laevis.

Korotkova DD , Gantsova EA , Goryashchenko AS , Eroshkin FM , Serova OV , Sokolov AS , Sharko F , Zhenilo SV , Martynova NY , Petrenko AG , Zaraisky AG , Deyev IE .

The orphan insulin receptor-related receptor (IRR) encoded by insrr gene is the third member of the insulin receptor family, also including the insulin receptor (IR) and the insulin-like growth factor receptor (IGF-1R). IRR is the extracellular alkaline medium sensor. In mice, insrr is expressed only in small populations of cells in specific tissues, which contain extracorporeal liquids of extreme pH. In particular, IRR regulates the metabolic bicarbonate excess in the kidney. In contrast, the role of IRR during Xenopus laevis embryogenesis is unknown, although insrr is highly expressed in frog embryos. Here, we examined the insrr function during the Xenopus laevis early development by the morpholino-induced knockdown. We demonstrated that insrr downregulation leads to development retardation, which can be restored by the incubation of embryos in an alkaline medium. Using bulk RNA-seq of embryos at the middle neurula stage, we showed that insrr downregulation elicited a general shift of expression towards genes specifically expressed before and at the onset of gastrulation. At the same time, alkali treatment partially restored the expression of the neurula-specific genes. Thus, our results demonstrate the critical role of insrr in the regulation of the early development rate in Xenopus laevis.

PubMed ID: 36012515
PMC ID: PMC9409083
Article link: Int J Mol Sci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: des.1 des.2 eomes foxg1 ins insrr kit myc myl1 pax6 pitx2 twsg1
GO keywords: insulin receptor binding [+]
Morpholinos: insrr MO1 insrr MO2

Article Images: [+] show captions
References [+] :
Almouzni, Histone acetylation influences both gene expression and development of Xenopus laevis. 1994, Pubmed, Xenbase