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Biophys J 2023 Feb 21;1224:661-671. doi: 10.1016/j.bpj.2023.01.015.
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Ion channel thermodynamics studied with temperature jumps measured at the cell membrane.

Bassetto CAZ , Pinto BI , Latorre R , Bezanilla F .

Perturbing the temperature of a system modifies its energy landscape, thus providing a ubiquitous tool to understand biological processes. Here, we developed a framework to generate sudden temperature jumps (Tjumps) and sustained temperature steps (Tsteps) to study the temperature dependence of membrane proteins under voltage clamp while measuring the membrane temperature. Utilizing the melanin under the Xenopus laevis oocytes membrane as a photothermal transducer, we achieved short Tjumps up to 9°C in less than 1.5 ms and constant Tsteps for durations up to 150 ms. We followed the temperature at the membrane with sub-ms time resolution by measuring the time course of membrane capacitance, which is linearly related to temperature. We applied Tjumps in Kir1.1 isoform b, which reveals a highly temperature-sensitive blockage relief, and characterized the effects of Tsteps on the temperature-sensitive channels TRPM8 and TRPV1. These newly developed approaches provide a general tool to study membrane protein thermodynamics.

PubMed ID: 36654507
PMC ID: PMC9989882
Article link: Biophys J
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: trpm8 trpv1
GO keywords: ion channel activity [+]

Article Images: [+] show captions
References [+] :
Baez, Gating of thermally activated channels. 2014, Pubmed