XB-ART-59722Development 2023 Feb 15;1504:. doi: 10.1242/dev.201309.
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Production and characterization of monoclonal antibodies to Xenopus proteins.
Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple Xenopus proteins and their validation in various experimental approaches. These novel antibodies are now available to the research community through the Developmental Study Hybridoma Bank (DSHB).
PubMed ID: 36789951
PMC ID: PMC10112901
Article link: Development
Species referenced: Xenopus laevis
Genes referenced: adam11 adam13 adam19 adam9 eya1 foxd3 mcrs1 myc prdm12 rpn1 six1 six3 snai2 sox2 sox3 tbxt twist1
GO keywords: antigen binding
Antibodies: FLAG Ab6 FLAG Ab7
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