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Front Cell Dev Biol 2023 Jan 01;11:1274788. doi: 10.3389/fcell.2023.1274788.
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Zmym4 is required for early cranial gene expression and craniofacial cartilage formation.

Jourdeuil K , Neilson KM , Cousin H , Tavares ALP , Majumdar HD , Alfandari D , Moody SA .

Introduction: The Six1 transcription factor plays important roles in the development of cranial sensory organs, and point mutations underlie craniofacial birth defects. Because Six1's transcriptional activity can be modulated by interacting proteins, we previously screened for candidate interactors and identified zinc-finger MYM-containing protein 4 (Zmym4) by its inclusion of a few domains with a bona fide cofactor, Sine oculis binding protein (Sobp). Although Zmym4 has been implicated in regulating early brain development and certain cancers, its role in craniofacial development has not previously been described. Methods: We used co-immunoprecipitation and luciferase-reporter assays in cultured cells to test interactions between Zmym4 and Six1. We used knock-down and overexpression of Zmym4 in embryos to test for its effects on early ectodermal gene expression, neural crest migration and craniofacial cartilage formation. Results: We found no evidence that Zmym4 physically or transcriptionally interacts with Six1 in cultured cells. Nonetheless, knockdown of endogenous Zmym4 in embryos resulted in altered early cranial gene expression, including those expressed in the neural border, neural plate, neural crest and preplacodal ectoderm. Experimentally increasing Zmym4 levels had minor effects on neural border or neural plate genes, but altered the expression of neural crest and preplacodal genes. At larval stages, genes expressed in the otic vesicle and branchial arches showed reduced expression in Zmym4 morphants. Although we did not detect defects in neural crest migration into the branchial arches, loss of Zmym4 resulted in aberrant morphology of several craniofacial cartilages. Discussion: Although Zmym4 does not appear to function as a Six1 transcriptional cofactor, it plays an important role in regulating the expression of embryonic cranial genes in tissues critical for normal craniofacial development.

PubMed ID: 37854072
PMC ID: PMC10579616
Article link: Front Cell Dev Biol
Grant support: [+]

Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: dlx5 eya1 foxd3 irx1 mcrs1 msx1 myc pa2g4 pax3 six1 sobp sox11 sox2 sox9 tbl1x tbx1 tfap2a tle4 zmym4
GO keywords: brain development [+]
Morpholinos: zmym4 MO1 zmym4 MO2

Disease Ontology terms: branchiootorenal syndrome [+]

Article Images: [+] show captions
References [+] :
Acampora, Craniofacial, vestibular and bone defects in mice lacking the Distal-less-related gene Dlx5. 1999, Pubmed