Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-60477
Ecotoxicol Environ Saf 2024 Jan 01;269:115820. doi: 10.1016/j.ecoenv.2023.115820.
Show Gene links Show Anatomy links

Early life exposure to perfluorooctanesulfonate (PFOS) impacts vital biological processes in Xenopus laevis: Integrated morphometric and transcriptomic analyses.

Ismail T , Lee HK , Lee H , Kim Y , Kim E , Lee JY , Kim KB , Ryu HY , Cho DH , Kwon TK , Park TJ , Kwon T , Lee HS .


Abstract
Perfluorooctanesulfonate (PFOS) is a ubiquitous environmental pollutant associated with increasing health concerns and environmental hazards. Toxicological analyses of PFOS exposure are hampered by large interspecies variations and limited studies on the mechanistic details of PFOS-induced toxicity. We investigated the effects of PFOS exposure on Xenopus laevis embryos based on the reported developmental effects in zebrafish. X. laevis was selected to further our understanding of interspecies variation in response to PFOS, and we built upon previous studies by including transcriptomics and an assessment of ciliogenic effects. Midblastula-stage X. laevis embryos were exposed to PFOS using the frog embryo teratogenesis assay Xenopus (FETAX). Results showed teratogenic effects of PFOS in a time- and dose-dependent manner. The morphological abnormalities of skeleton deformities, a small head, and a miscoiled gut were associated with changes in gene expression evidenced by whole-mount in situ hybridization and transcriptomics. The transcriptomic profile of PFOS-exposed embryos indicated the perturbation in the expression of genes associated with cell death, and downregulation in adenosine triphosphate (ATP) biosynthesis. Moreover, we observed the effects of PFOS exposure on cilia development as a reduction in the number of multiciliated cells and changes in the directionality and velocity of the cilia-driven flow. Collectively, these data broaden the molecular understanding of PFOS-induced developmental effects, whereby ciliary dysfunction and disrupted ATP synthesis are implicated as the probable modes of action of embryotoxicity. Furthermore, our findings present a new challenge to understand the links between PFOS-induced developmental toxicity and vital biological processes.

PubMed ID: 38103469
Article link: Ecotoxicol Environ Saf


Species referenced: Xenopus laevis
Genes referenced: aif1l amy2a arg1 ass1 c5ar1 cel cela1 chad col8a2 cpb1 cxcl8a darmin ddit4l fabp2 foxd3 gbp1 ldlrap1 mmp1 myo1f nkx2-5 olfm4 otc pax1 pax6 pla2g1b plg prss1 rho slc14a2 sox3 tnfrsf12a tuba4b uts2
Antibodies: Tuba4a Ab4

Phenotypes: Xla Wt +PFOS (Fig. 1 A) [+]

Article Images: [+] show captions