XB-ART-9093
EMBO J
2001 May 01;209:2315-25. doi: 10.1093/emboj/20.9.2315.
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A proline-rich protein binds to the localization element of Xenopus Vg1 mRNA and to ligands involved in actin polymerization.
Zhao WM
,
Jiang C
,
Kroll TT
,
Huber PW
.
Abstract
A 340 nucleotide element within the 3' untranslated region of Vg1 mRNA determines its localization to the vegetal cortex of Xenopus oocytes. To identify protein factors that bind to this region, we screened a cDNA expression library with an RNA probe containing this sequence. Five independent isolates encoded a protein (designated Prrp for proline-rich RNA binding protein) having two RNP domains followed by multiple polyproline segments. Prrp and Vg1 mRNAs are co-localized to the vegetal cortex of stage IV oocytes, substantiating an interaction between the two in vivo. Prrp also associates with VegT mRNA, which like Vg1 mRNA uses the late localization pathway, but not with Xcat-2 or Xwnt-11 mRNAs, which use the early pathway. The proline-rich domain of Prrp interacts with profilin, a protein that promotes actin polymerization. Prrp can also associate with the EVH1 domain of Mena, another microfilament-associated protein. Since the anchoring of Vg1 mRNA to the vegetal cortex is actin dependent, one function of Prrp may be to facilitate local actin polymerization, representing a novel function for an RNA binding protein.
PubMed ID: 11331596
PMC ID: PMC125447
Article link: EMBO J
Species referenced: Xenopus
Genes referenced: actl6a gdf1 pfn1 prlh vegt
References [+] :
Ahern-Djamali,
Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled.
1999, Pubmed
Ahern-Djamali, Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. 1999, Pubmed
Ashley, FMR1 protein: conserved RNP family domains and selective RNA binding. 1993, Pubmed
Bashirullah, RNA localization in development. 1998, Pubmed , Xenbase
Bassell, mRNA and cytoskeletal filaments. 1997, Pubmed
Beach, Localization and anchoring of mRNA in budding yeast. 1999, Pubmed
Beckerle, Spatial control of actin filament assembly: lessons from Listeria. 1998, Pubmed
Bedford, FBP WW domains and the Abl SH3 domain bind to a specific class of proline-rich ligands. 1997, Pubmed
Bergsten, Role for mRNA localization in translational activation but not spatial restriction of nanos RNA. 1999, Pubmed
Blanchoin, Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin. 1998, Pubmed
Breeden, Regulation of the yeast HO gene. 1985, Pubmed
Burd, Conserved structures and diversity of functions of RNA-binding proteins. 1994, Pubmed
Chien, The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. 1991, Pubmed
Chomczynski, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 1987, Pubmed
Cote, A Xenopus protein related to hnRNP I has a role in cytoplasmic RNA localization. 1999, Pubmed , Xenbase
Dale, Secretion and mesoderm-inducing activity of the TGF-beta-related domain of Xenopus Vg1. 1993, Pubmed , Xenbase
Deshler, Localization of Xenopus Vg1 mRNA by Vera protein and the endoplasmic reticulum. 1997, Pubmed , Xenbase
Deshler, A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates. 1998, Pubmed , Xenbase
Dumont, Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. 1972, Pubmed , Xenbase
Elisha, Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes. 1995, Pubmed , Xenbase
Erdélyi, Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization. 1995, Pubmed
Evangelista, Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. 1997, Pubmed
Fan, HNS, a nuclear-cytoplasmic shuttling sequence in HuR. 1998, Pubmed , Xenbase
Fedorov, Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli. 1994, Pubmed
Ferrandon, Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner. 1994, Pubmed
Forristall, Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2. 1995, Pubmed , Xenbase
Frazier, Actin cytoskeleton: are FH proteins local organizers? 1997, Pubmed
Gertler, enabled, a dosage-sensitive suppressor of mutations in the Drosophila Abl tyrosine kinase, encodes an Abl substrate with SH3 domain-binding properties. 1995, Pubmed
Gertler, Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. 1996, Pubmed
Goldschmidt-Clermont, The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells. 1992, Pubmed
Golsteyn, Structural and functional similarities between the human cytoskeletal protein zyxin and the ActA protein of Listeria monocytogenes. 1997, Pubmed
Haffner, Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP. 1995, Pubmed
Hall, Interaction of N-terminal domain of U1A protein with an RNA stem/loop. 1992, Pubmed
Havin, RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization. 1998, Pubmed , Xenbase
Hoek, hnRNP A2 selectively binds the cytoplasmic transport sequence of myelin basic protein mRNA. 1998, Pubmed , Xenbase
Houston, A Xenopus DAZ-like gene encodes an RNA component of germ plasm and is a functional homologue of Drosophila boule. 1998, Pubmed , Xenbase
Kang, Profilin promotes barbed-end actin filament assembly without lowering the critical concentration. 1999, Pubmed
Kay, The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains. 2000, Pubmed
Kloc, Two distinct pathways for the localization of RNAs at the vegetal cortex in Xenopus oocytes. 1995, Pubmed , Xenbase
Laurent, Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. 1999, Pubmed
Long, Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. 1997, Pubmed
Maines, Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule. 1999, Pubmed
Maruyama, cRACE: a simple method for identification of the 5' end of mRNAs. 1995, Pubmed
Melton, Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes. , Pubmed , Xenbase
Michael, The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hnRNP K protein. 1997, Pubmed , Xenbase
Michael, A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway. 1995, Pubmed
Milligan, Synthesis of small RNAs using T7 RNA polymerase. 1989, Pubmed
Mowry, Complex formation between stage-specific oocyte factors and a Xenopus mRNA localization element. 1996, Pubmed , Xenbase
Mowry, RNA sorting in Xenopus oocytes and embryos. 1999, Pubmed , Xenbase
Mowry, Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes. 1992, Pubmed , Xenbase
Nakielny, Transport of proteins and RNAs in and out of the nucleus. 1999, Pubmed
Niebuhr, A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. 1997, Pubmed
Pantaloni, How profilin promotes actin filament assembly in the presence of thymosin beta 4. 1993, Pubmed
Reinhard, The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. 1995, Pubmed
Ren, Identification of a ten-amino acid proline-rich SH3 binding site. 1993, Pubmed
Rickles, Phage display selection of ligand residues important for Src homology 3 domain binding specificity. 1995, Pubmed
Ross, Characterization of a beta-actin mRNA zipcode-binding protein. 1997, Pubmed
Schlüter, Profilins as regulators of actin dynamics. 1997, Pubmed
Schroeder, Xenopus poly (A) binding protein maternal RNA is localized during oogenesis and associated with large complexes in blastula. 1996, Pubmed , Xenbase
Sheibani, Direct use of synthetic peptides for antiserum production. 1998, Pubmed
Shen, Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila. 1998, Pubmed
Shyu, The double lives of shuttling mRNA binding proteins. 2000, Pubmed
Singh, Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. 1988, Pubmed
Siomi, Functional conservation of the transportin nuclear import pathway in divergent organisms. 1998, Pubmed , Xenbase
Sparks, Distinct ligand preferences of Src homology 3 domains from Src, Yes, Abl, Cortactin, p53bp2, PLCgamma, Crk, and Grb2. 1996, Pubmed
St Johnston, Staufen, a gene required to localize maternal RNAs in the Drosophila egg. 1991, Pubmed
Sundell, Requirement of microfilaments in sorting of actin messenger RNA. 1991, Pubmed
Thomsen, Processed Vg1 protein is an axial mesoderm inducer in Xenopus. 1993, Pubmed , Xenbase
Tsui, Association of the mouse infertility factor DAZL1 with actively translating polyribosomes. 2000, Pubmed
Tsui, Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1. 2000, Pubmed
Turner, Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate. 1994, Pubmed , Xenbase
Wasserman, FH proteins as cytoskeletal organizers. 1998, Pubmed
Weeks, A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta. 1987, Pubmed , Xenbase
Wilhelm, Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes. 2000, Pubmed
Wilson, Assembly of AUF1 oligomers on U-rich RNA targets by sequential dimer association. 1999, Pubmed
Wolven, In vivo importance of actin nucleotide exchange catalyzed by profilin. 2000, Pubmed
Yisraeli, The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes. 1988, Pubmed , Xenbase
Yisraeli, A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA. 1990, Pubmed , Xenbase
Zhang, Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning. 1996, Pubmed , Xenbase
Zhang, Vg1 RBP intracellular distribution and evolutionarily conserved expression at multiple stages during development. 1999, Pubmed , Xenbase