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Mech Dev
2000 Dec 01;991-2:163-6. doi: 10.1016/s0925-4773(00)00468-8.
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Characterization and developmental expression of xSim, a Xenopus bHLH/PAS gene related to the Drosophila neurogenic master gene single-minded.
Coumailleau P
,
Penrad-Mobayed M
,
Lecomte C
,
Bollérot K
,
Simon F
,
Poellinger L
,
Angelier N
.
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We have isolated a novel gene from Xenopus, denominated xSim, which encodes a protein of 760 amino acids containing a basic helix-loop-helix (bHLH) motif contiguous to a PAS domain characteristic of an emerging family of transcriptional regulators so called bHLH/PAS. xSim shares a strong amino acid sequence identity with the Drosophila Single-minded (dSim) and with the murine Sim1 and Sim2 proteins. Phylogenetic analysis reveals that xSim gene is an ortholog gene of the mSim2 gene. Spatio-temporal analysis shows a maternal and a zygotic expression of xSim throughout early Xenopus development. In situ hybridization assays reveal that the transcripts are enriched in the animal hemisphere until blastula stage and extend to the marginal zone at early gastrula stage. As development proceeds, xSim is mainly restricted to the central nervous system.
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11091086
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Fig. 2. (A) In vitro translation of xSim. Wild-type xSim protein is expressed by in vitro translation in rabbit reticulysate lysate and assessed by SDS-PAGE and autoradiography after incorporation of 35S methionine. (B) Temporal expression analysis of xSim during Xenopus embryogenesis analyzed by RT-PCR. Embryonic stages tested (Nieuwkoop and Faber, 1967) are indicated above each column. Specific xSim primers (see (Fig. 1) were chosen in the C-terminal region. Signals obtained for xSim was compared with those obtained for ODC. (C) Spatial expression of xSim analyzed by whole-mount and sections in situ hybridizations. (a) Lateral view of stage 6 embryo, vegetal pole is at the bottom. (b) Stage 7 embryo, animal view. (c) Stage 10.5. Dorso-vegetal view. (d) Stage 20. Lateral view, anterior to the left. (e) Stage 35, tail bud embryo, lateral view. (f) Stage 8. Meridian section. (g) Stage 10. Sagittal section. Arrow head indicates the blastopore. (h) Stage 18. Transverse section. (i,j) Stage 30. Transverse sections. (k) Stage 30. Sagittal section.
sim2 (single-minded homolog 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 6, lateral view, animal pole up.
sim2 (single-minded homolog 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 7, animal view.
sim2 (single-minded homolog 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, vegetal view.
sim2 (single-minded homolog 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 20, lateral view, anteriorleft, dorsal up.
sim2 (single-minded homolog 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view, anteriorleft, dorsal up.
