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Antibody Name: Vim Ab1 Common Name: Vimentin RRID: AB_528507 Synonyms: 14 h7, 14h7 Antigens Gene: vim Clone Type: Monoclonal Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number	14h7
Purification	Affinity Purification
Isotype	IgG1
Host Organism	mouse
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity	chicken, mouse
Description:	Supplied by DSHB, cat#14h7. Contributed to DSHB by Klymkowsky Lab. While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively. Cite this: (DSHB Cat# 14h7, RRID:AB_528507). please email citation to DSHB.
Immunogen
Name	Vimentin
Type	polypeptide
Post Translational Modifications	None
Source Organism	frog
Description	Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments). This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.
Sequence
Reported Usage
immunohistochemistry
western blot	Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa
immunofluorescence
Publications
???displayAntibody.papersFirst???	Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos., Development 1987
???displayAntibody.papersRecent???	Cellular response to spinal cord injury in regenerative and non-regenerative stages in Xenopus laevis., Neural Dev 2021
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