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XB-ANTIBODY-22041796
Antibody Name:
Rdd Ab1
Common Name: anti-rdd RRID: Synonyms: Rdd3, Rdd4
Source: |
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Antibody | |
Clone Number | |
Purification | Affinity Purification |
Isotype | IgG |
Host Organism | rabbit |
Xenopus Reactivity | X. laevis |
Non-Xenopus Reactivity | |
Description: | Custom Antibody against peptide common to Rdd3 and Rdd4 developed by Mitsugu lab. |
Immunogen | |
Name | rdd |
Type | synthetic sequence |
Post Translational Modifications | None |
Source Organism | frog |
Description | A common amino acid sequence in Rdd3 and Rdd4, SQCPPSQEQRCPLNQFWE (amino acids 149-166 of Rdd3, amino acids 214-231 of Rdd4), was selected as the antigenic and hydrophilic site to produce anti-Rdd peptide serum. Rdd peptide was synthesized and conjugated with keyhole limpet hemocyanin. |
Sequence |
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Reported Usage | |
immunohistochemistry | To detect endogenous Rdd proteins, albino embryos were incubated with anti-rdd antibody as a first antibody and were incubated with AP-conjugated anti-rabbit IgG as a second antibody. The reaction was visualized in NBT/BCIP solution. Rdd pep tides (20 µg/ml) were added with anti-Rdd serum for an antibody absorption experiment. |
Publications | |
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Expression and localization of Rdd proteins in Xenopus embryo., Anat Cell Biol 2014 ![]() |
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