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Summary Images Attributions Wiki Source
XB-ANTIBODY-22064444

Antibody Name: Tuba4a Ab20

Common Name: anti alpha tubulin

RRID: AB_579793
Synonyms: AA4.3, TBA1 Antibody

Antigens
Gene: tuba4b

Clone Type: Monoclonal
Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number AA4.3
Purification unknown how antibody is supplied
Isotype IgG1
Host Organism mouse
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity fruitfly, human, mouse, rat, worm, zebrafish
Description: Citation: (DSHB Cat# AA4.3, RRID:AB_579793). Please email citation to DSHB.
Immunogen
Name flagellar axoneme
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description Predicted: 50 kDa; Apparent: 55 kDa. Initial characterization [PMID 6363422] performed on Naegleria gruberi tubulins suggested that this antibody target was beta-tubulin. Further characterization [PMID 3654753] demonstrated that the target was alpha-tubulin. This antibody may recognize more than one alpha-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Sequence
Reported Usage
enzyme-linked immunosorbent assay
immunohistochemistry
immunoprecipitation
western blot
Publications
???displayAntibody.papersFirst??? Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates., J Cell Biol 1984  
???displayAntibody.papersRecent??? Protein turnover dynamics suggest a diffusion-to-capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins., Mol Biol Cell 2021     
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