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XB-ANTIBODY-29072242
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Antibody Name:
FLAG Ab6
Common Name: 12C6c RRID: AB_2890618 Synonyms:
Source: Developmental Studies Hybridoma Bank |
1/3 
| Antibody | |
| Clone Number | DA12C6 |
| Purification | Affinity Purification |
| Isotype | IgG2 |
| Host Organism | mouse |
| Xenopus Reactivity | X. tropicalis, X. laevis |
| Non-Xenopus Reactivity | chicken, fruitfly, human, mouse, rat, worm, yeast, zebrafish |
| Description: | This FLAG antibody Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody. 12C6c was deposited to the DSHB by Dom Alfandari, University of Massachusetts, Amherst. Citation (DSHB Hybridoma Product 12C6c; RRID:AB_2890618 ) |
| Immunogen | |
| Name | FLAG-tag |
| Type | polypeptide |
| Post Translational Modifications | None |
| Source Organism | Other |
| Description | Immunogen: Recombinant protein Flag Octapeptide Epitope Location or Sequence: DYKDDDDK |
| Sequence |
[+]
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| Reported Usage | |
| electrophoretic mobility shift assay | |
| immunofluorescence | |
| immunoprecipitation | |
| western blot | To remove WB background staining, wash 3X with TBST plus 500 mM NaCl (High salt TBST). Similarly, to remove background IF background staining, 3 high salt washes in PBS followed by 1 wash with 0.5% Triton is recommended. |
| Used For Epitope Tagging | Yes |
| Publications | |
| ???displayAntibody.papersFirst??? |
Production and characterization of monoclonal antibodies to Xenopus proteins., Development 2023  |
| ???displayAntibody.papersRecent??? | |
| ???displayAntibody.papersAll??? | |
