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XB-ANTIBODY-29225883
Antibody Name:
Smad2 Ab12
Common Name: SMAD2 XP rabbit mAb5339 RRID: Synonyms: 5339
Source: |
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Antibody | |
Clone Number | |
Purification | Affinity Purification |
Isotype | IgG |
Host Organism | rabbit |
Xenopus Reactivity | X. laevis |
Non-Xenopus Reactivity | human, monkey, mouse, rat |
Description: | SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-beta signals from the cell surface into the nucleus. SMAD2 (D43B4) XP Rabbit mAb detects endogenous levels of total SMAD2 protein. This antibody does not cross-react with SMAD3. |
Immunogen | |
Name | Smad2 |
Type | synthetic sequence |
Post Translational Modifications | unknown post translational modifications |
Source Organism | mouse |
Description | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse SMAD2 protein. |
Sequence | |
Reported Usage | |
chromatin immunoprecipitation | 1:50 For optimal ChIP results, use 10 nl of antibody and 10ng of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP Enzymatic Chromatin IP Kits. |
western blot | 1:1000 |
immunofluorescence | 1:50-1:200 |
immunoprecipitation | 1:50 |
flow cytometry | 1:100-1:400 |
Publications | |
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Artesunate disrupts germ layer formation by inhibiting BMP signaling pathway., Anim Cells Syst (Seoul) 2025 ![]() |
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