Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.

Summary Images Attributions Wiki Source
XB-ANTIBODY-29225883

Antibody Name: Smad2 Ab12

Common Name: SMAD2 XP rabbit mAb5339

RRID:
Synonyms: 5339

Antigens

Clone Type: Monoclonal
Source:

Antibody
Clone Number
Purification Affinity Purification
Isotype IgG
Host Organism rabbit
Xenopus Reactivity X. laevis
Non-Xenopus Reactivity human, monkey, mouse, rat
Description: SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-beta signals from the cell surface into the nucleus. SMAD2 (D43B4) XP Rabbit mAb detects endogenous levels of total SMAD2 protein. This antibody does not cross-react with SMAD3.
Immunogen
Name Smad2
Type synthetic sequence
Post Translational Modifications unknown post translational modifications
Source Organism mouse
Description Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse SMAD2 protein.
Sequence
Reported Usage
chromatin immunoprecipitation 1:50 For optimal ChIP results, use 10 nl of antibody and 10ng of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP Enzymatic Chromatin IP Kits.
western blot 1:1000
immunofluorescence 1:50-1:200
immunoprecipitation 1:50
flow cytometry 1:100-1:400
Publications
???displayAntibody.papersFirst??? Artesunate disrupts germ layer formation by inhibiting BMP signaling pathway., Anim Cells Syst (Seoul) 2025     
???displayAntibody.papersRecent???
???displayAntibody.papersAll???