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XB-ANTIBODY-22201260

Antibody Name: SLC12A1 Ab1

Common Name: Na-K-Cl cotransporter

RRID: AB_528406
Synonyms:

Antigens
Gene: slc12a1

Clone Type: Monoclonal
Source:

Antibody
Clone Number T4
Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity X. tropicalis, X. laevis
Non-Xenopus Reactivity cow, dog, human, monkey, mouse, pig, rabbit, rat
Description: Citation: (DSHB Cat# t4, RRID:AB_528406). Please send all citations to DSHB. Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T4 recognizes NKCC1 and NKCC2 on western blots and by IF, but is weak for NKCC2 by IF (PMID: 8572179). Paraffin embedment of ethanol fixed tissue.
Immunogen
Name Recombinant Na-K-Cl cotransporter protein
Type polypeptide
Post Translational Modifications None
Source Organism human
Description Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein
Sequence [+]
Reported Usage
immunohistochemistry
immunoprecipitation
western blot
immunofluorescence
Publications
First: Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies., Am J Physiol 1995  
Most recent:
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