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XB-ANTIBODY-25900437

Antibody Name: Vim Ab5

Common Name: Anti-Vimentin antibody (SP20)

RRID: AB_443435
Synonyms: ab16700

Antigens
Gene: vim

Clone Type: Monoclonal
Source: Abcam

Antibody
Clone Number SP20
Purification Affinity Purification
Isotype IgG
Host Organism rabbit
Xenopus Reactivity X. laevis
Non-Xenopus Reactivity human
Description: Rabbit monoclonal [SP20] to Vimentin.Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Cite: Abcam, #ab16700
Immunogen
Name
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminu
Sequence
Reported Usage
immunofluorescence 1/1000
western blot Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
flow cytometry 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
immunohistochemistry 1/200. Antigen Retrieval: None
Publications
First: A Focal Impact Model of Traumatic Brain Injury in Xenopus Tadpoles Reveals Behavioral Alterations, Neuroinflammation, and an Astroglial Response., Int J Mol Sci 2022                          
Most recent: β-Catenin and SOX2 Interaction Regulate Visual Experience-Dependent Cell Homeostasis in the Developing Xenopus Thalamus., Int J Mol Sci 2023                  
View All Papers