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Summary Attributions Wiki
XB-GRNA-23659118
gRNA Name: parm1 gRNA1
Synonyms:
Genomic Target: parm1

PAM Sequence: GGG
Sequence: 5' GAACAAGATTATGGCTCCTT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' AAGGAGCCATAATCTTGTTC 3'
   ||||||||||||||||||||
3' TTCCTCGGTATTAGAACAAG 5'
 
5' AAGGAGCCATAATCTTGGTC 3'
   ||||||||||||||||| ||
3' TTCCTCGGTATTAGAACAAG 5'
 
5' AAGGAGCCATAATCTTGATC 3'
   ||||||||||||||||| ||
3' TTCCTCGGTATTAGAACAAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr1L:84934621..84934640 19/20 Antisense target parm1.L
Chr1S:68746576..68746595 19/20 Antisense target parm1.S
Chr6S:34388846..34388865 18/20 Sense off-target skap2.S
Chr6L:134280845..134280864 17/20 Antisense off-target ralyl.L
Chr1L:225407632..225407651 17/20 Antisense off-target setbp1.L
Chr9_10S:39456345..39456364 16/20 Antisense off-target mrps7.S
Chr2S:43621626..43621645 16/20 Antisense off-target plxna2.S
Chr6S:128670159..128670178 18/20 Sense unknown
Chr3S:91838599..91838618 17/20 Antisense unknown
Chr9_10L:25611842..25611861 17/20 Antisense unknown
Chr1S:91185447..91185466 17/20 Sense unknown
Chr5S:108421847..108421866 16/20 Antisense unknown
tropicalis 10.0 Chr1:79605352..79605371 20/20 Antisense target parm1
Chr3:90725012..90725031 16/20 Antisense off-target pcasp3
Chr1:115454217..115454236 18/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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