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XB-GRNA-23659318
gRNA Name:
bace1 gRNA1
Synonyms:
Genomic Target: bace1
PAM Sequence: TGG
Sequence: 5' AGGTGAGCTAGGCACAGATTTGG 3'
Synonyms:
Genomic Target: bace1
PAM Sequence: TGG
Sequence: 5' AGGTGAGCTAGGCACAGATTTGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | 23/23 | 22/23 | ||||
genome
gRNA
|
5' CCAAATCTGTGCCTAGCTCACCT 3' ||||||||||||||||||||||| 3' GGTTTAGACACGGATCGAGTGGA 5' | 5' CCAAATCTGTGCCTAGCTCACCT 3' ||||||||||||||||||||||| 3' GGTTTAGACACGGATCGAGTGGA 5' | 5' CCAGATCTGTGCCTAGCTCACCT 3' ||| ||||||||||||||||||| 3' GGTTTAGACACGGATCGAGTGGA 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Genomic Target | |||
| laevis 10.1 | Chr7L:83516452..83516474 | 23/23 | Sense | target | bace1.L | ||
| Chr7S:71394107..71394129 | 22/23 | Sense | target | bace1.S | |||
| Chr2S:42305067..42305089 | 20/23 | Antisense | off-target | lamb3.S | |||
| Chr9_10L:80663768..80663790 | 19/23 | Sense | off-target | itga4.L | tropicalis 10.0 | Chr7:81941116..81941138 | 23/23 | Sense | bace1 |
| Chr4:30747730..30747752 | 19/23 | Antisense | chid1 | ||||
| Chr5:28925737..28925759 | 19/23 | Sense | macrod2 | ||||
| Chr6:86707193..86707215 | 19/23 | ||||||
| Chr8:88357631..88357653 | 19/23 | ||||||
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