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XB-gRNA-23659381
gRNA Name:
glis3 gRNA1
Synonyms:
Genomic Target: glis3
PAM Sequence: AGG
Sequence: 5' CCCCCCATTAGAGTCCATACAGG 3'
Synonyms:
Genomic Target: glis3
PAM Sequence: AGG
Sequence: 5' CCCCCCATTAGAGTCCATACAGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 23/23 | 23/23 | 23/23 | ||||
| genomic gRNA |
5' CCTGTATGGACTCTAATGGGGGG 3' ||||||||||||||||||||||| 3' GGACATACCTGAGATTACCCCCC 5' | 5' CCTGTATGGACTCTAATGGGGGG 3' ||||||||||||||||||||||| 3' GGACATACCTGAGATTACCCCCC 5' | 5' CCTGTATGGACTCTAATGGGGGG 3' ||||||||||||||||||||||| 3' GGACATACCTGAGATTACCCCCC 5' | ||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | ||
| laevis 9.2 | Scaffold58:906796..906818 | 23/23 | Sense | target | glis3.S | ||
| chr1L:125586838..125586860 | 23/23 | Sense | target | glis3.L | |||
| chr1L:77476713..77476735 | 19/23 | Sense | off-target | idua.L | |||
| chr2L:177481465..177481487 | 20/23 | Antisense | off-target | sytl2.L | |||
| chr8L:91644429..91644451 | 19/23 | Sense | off-target | npas3.L | |||
| chr9_10S:55684350..55684372 | 19/23 | Antisense | off-target | baz2b.S | |||
| chr1S:11310228..11310250 | 20/23 | unknown | tropicalis 10.0 | Chr1:127473827..127473849 | 23/23 | Sense | target | glis3 |
| Chr5:150378016..150378038 | 20/23 | unknown | |||||
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