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Summary Attributions Wiki
XB-GRNA-23659413
gRNA Name: nhlh1 gRNA1
Synonyms: trop nhlh1 T1
Genomic Target: nhlh1

PAM Sequence: TGG
Sequence: 5' GCCAGTGGGATCAGCTTCTGTGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 21/23 19/23
genome
gRNA
 
5' CCACAGAAGCTGATCCCACTGGC 3'
   |||||||||||||||||||||||
3' GGTGTCTTCGACTAGGGTGACCG 5'
 
5' CCACACAAGCTGATCCCACTGCC 3'
   ||||| ||||||||||||||| |
3' GGTGTCTTCGACTAGGGTGACCG 5'
 
5' CCGCACAAGCTGATCCCACTGAT 3'
   || || |||||||||||||||  
3' GGTGTCTTCGACTAGGGTGACCG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr8L:112293297..112293319 21/23 Antisense target nhlh1.L
chr8S:94127586..94127608 19/23 Antisense target nhlh1.S
chr3L:122358573..122358595 21/23 Antisense off-target fam160b2.L
Scaffold25:1825466..1825488 19/23 Antisense off-target zc3h18.S
chr2L:151880413..151880435 19/23 Sense off-target rcbtb1.L
chr6S:2807425..2807447 20/23 Antisense unknown
chr4S:19193912..19193934 19/23 Sense unknown
chr4S:5039285..5039307 19/23 Sense unknown
chr7L:22893845..22893867 19/23 Sense unknown
tropicalis 10.0 Chr8:137228384..137228406 23/23 Antisense target nhlh1
Chr3:92562723..92562745 20/23 Sense off-target insyn1
Chr3:104273859..104273881 20/23 Antisense off-target fam227b
Chr4:150779958..150779980 19/23 Sense off-target tkt
Chr5:150975872..150975894 19/23 Antisense off-target xkr6
Chr7:6150584..6150606 19/23 Sense off-target eif3a
Chr10:10415253..10415275 19/23 Sense off-target cacnb1
Chr7:94531058..94531080 19/23 Antisense off-target LOC101734646
Chr3:43002102..43002124 19/23 Antisense unknown
Publications
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