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Summary Attributions Wiki
XB-gRNA-23659413
gRNA Name: nhlh1 gRNA1
Synonyms: trop nhlh1 T1
Genomic Target: nhlh1

PAM Sequence: TGG
Sequence: 5' GCCAGTGGGATCAGCTTCTGTGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 21/23 19/23
genome
gRNA
 
5' CCACAGAAGCTGATCCCACTGGC 3'
   |||||||||||||||||||||||
3' GGTGTCTTCGACTAGGGTGACCG 5'
 
5' CCACACAAGCTGATCCCACTGCC 3'
   ||||| ||||||||||||||| |
3' GGTGTCTTCGACTAGGGTGACCG 5'
 
5' CCGCACAAGCTGATCCCACTGAT 3'
   || || |||||||||||||||  
3' GGTGTCTTCGACTAGGGTGACCG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr8L:127427251..127427273 21/23 Sense target nhlh1.L
Chr8S:99163265..99163287 19/23 Sense target nhlh1.S
Chr3L:137918416..137918438 21/23 Antisense off-target fhip2b.L
Chr2L:157785514..157785536 19/23 Antisense off-target rcbtb1.L
Chr4S:48823617..48823639 19/23 Antisense off-target zc3h18.S
Chr6S:3218533..3218555 20/23 unknown
Chr4S:19783626..19783648 19/23 unknown
Chr4S:5206367..5206389 19/23 unknown
Chr7L:22839819..22839841 19/23 unknown
tropicalis 10.0 Chr8:137228384..137228406 23/23 Sense target nhlh1
Chr3:92562723..92562745 20/23 Antisense off-target insyn1
Chr3:104273859..104273881 20/23 Sense off-target fam227b
Chr4:150779958..150779980 19/23 Sense off-target tkt
Chr5:150975872..150975894 19/23 Antisense off-target xkr6
Chr7:6150584..6150606 19/23 Antisense off-target eif3a
Chr10:10415253..10415275 19/23 Sense off-target cacnb1
Chr7:94531058..94531080 19/23 Antisense off-target LOC101734646
Chr3:43002102..43002124 19/23 unknown
Publications
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