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XB-GRNA-23659713
gRNA Name: pdx1 gRNA3
Synonyms: pdx1-T3
Genomic Target: pdx1

PAM Sequence: ggg
Sequence: 5' GGGAATGGCATTTGCTGGTG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' CACCAGCAAATGCCATTCCC 3'
   ||||||||||||||||||||
3' GTGGTCGTTTACGGTAAGGG 5'
 
5' CAACAGCAAATGCCATTCCC 3'
   || |||||||||||||||||
3' GTGGTCGTTTACGGTAAGGG 5'
 
5' CACCACCAAATGCCATTCCC 3'
   ||||| ||||||||||||||
3' GTGGTCGTTTACGGTAAGGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr2L:158779150..158779169 19/20 Sense target pdx1.L
chr2S:138317905..138317924 19/20 Sense target pdx1.S
chr8L:59945650..59945669 18/20 Sense off-target mlh3.L
chr5L:126620897..126620916 18/20 Sense off-target Xelaev18027889m
chr7S:74541684..74541703 17/20 Antisense off-target arhgef1.S
chr9_10L:16062681..16062700 17/20 Antisense off-target phactr3.L
chr9_10S:14683758..14683777 17/20 Antisense off-target phactr3.S
chr6L:152596671..152596690 17/20 Antisense off-target Xelaev18032476m
Scaffold113:71677..71696 16/20 Antisense off-target ubap2.L
chr9_10L:53033704..53033723 16/20 Sense off-target tmbim1.L
chr5L:75919854..75919873 16/20 Antisense off-target trappc3l.L
chr6S:81800490..81800509 16/20 Sense off-target LOC108695247 [provisional:ptprm]
chr9_10S:88772731..88772750 18/20 Antisense unknown
chr1S:100575216..100575235 17/20 Antisense unknown
chr2L:24200626..24200645 17/20 Sense unknown
chr3L:113338290..113338309 16/20 Sense unknown
chr6L:79546596..79546615 16/20 Antisense unknown
tropicalis 10.0 Chr2:155160010..155160029 20/20 Sense target pdx1
Chr7:49210950..49210969 17/20 Antisense off-target ldb3
Chr8:131743253..131743272 17/20 Sense off-target pear1
Chr4:105053597..105053616 18/20 Sense unknown
Chr3:63153919..63153938 17/20 Sense unknown
Chr1:55956110..55956129 16/20 Antisense unknown
Chr10:29689661..29689680 16/20 Antisense unknown
Chr9:37341569..37341588 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014     
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