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Summary Attributions Wiki
XB-GRNA-23659745
gRNA Name: tyr gRNA16
Synonyms: tyrb-T
Genomic Target: tyr

PAM Sequence: CCT
Sequence: 5' CACCAGCTCTGCTACGGGCC 3'



Genomic Target tropicalis laevis.S
Identities 18/20 20/20
genome
gRNA
 
5' GGCCAGTAGCAGAGCTGGTC 3'
   |||| |||||||||||||| 
3' CCGGGCATCGTCTCGACCAC 5'
 
5' GGCCCGTAGCAGAGCTGGTG 3'
   ||||||||||||||||||||
3' CCGGGCATCGTCTCGACCAC 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr2S:149899602..149899621 20/20 Antisense target tyr.S
chr2L:171564316..171564335 19/20 Antisense off-target grm5-like.L
tropicalis 10.0 Chr2:167828438..167828457 18/20 Antisense target tyr
Publications
???morpholino.displaySummary.papersFirst??? Targeted gene disruption in Xenopus laevis using CRISPR/Cas9., Cell Biosci 2015     
???morpholino.displaySummary.papersRecent??? Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique., Dev Growth Differ 2022     
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