Figure 2. Structural integrity of mitotic spindle is disrupted in the mspsP mutant. Whole mount preparations of larval central nervous system were prepared from mspsP and stained to reveal tubulin (green), DNA (red), and a centrosomal antigen, CP190 (blue). The focal planes shown display the overall spindle structure and CP190 staining for each mitotic figure. (a) Category I. Apparently normal bipolar spindle with CP190 at both poles. (b) Category II. One bipolar spindle and one smaller bipolar spindle (arrowhead). CP190 is found at both poles of larger spindle and is missing from one pole of the smaller one. (c) Category II. Two bipolar spindles with one shared pole. CP190 is found at both poles of one spindle, while it is absent from the unshared pole of the other spindle (arrowhead). (d) Category II. At least three chromosomes are associated with each of small bipolar spindles. One spindle has CP190 at both poles, another has the protein at one pole, and the other shows no CP190 at the poles. (e) Category IV. One bipolar spindle and a monopolar spindle. The bipolar spindle has CP190 at both poles. (f) Category III. Short disorganized microtubule bundles associated with a chromosome mass. No discrete CP190 staining is seen. (g) Frequencies of each category of spindle structure observed. Bar, 5 μm.
Image published in: Cullen CF et al. (1999)
© 1999 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license
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