Fig S2 DEFG

Azbazdar Y and De Robertis EM (2024)

Xla Wt + ctnnb1 MO

NF11.5 (RT-PCR)

Expression Phenotype
increased amount ventx1.2.L expression in ventro-lateral marginal zone absent chrd.L expression in dorsal marginal zone decreased amount sia1.L expression in dorsal marginal zone increased amount szl.L expression in ventral marginal zone

Fig.6 e

Sempou E et al. (2022)

Xtr Wt + K+ (20mM high)

NF10-12 (RNA-Seq)

Expression Phenotype

increased amount ventx1.2 expression in whole organism
increased amount atg13 expression in whole organism
increased amount eomes expression in whole organism
increased amount foxh1 expression in whole organism
increased amount pou5f3.3 expression in whole organism
increased amount rictor expression in whole organism
increased amount smad4 expression in whole organism
increased amount sox3 expression in whole organism

Fig. 7. a_c4,d, b_c4,e, c_c4,f

Sempou E et al. (2022)

Xtr Wt + K+ (20mM high)

NF10 (in situ hybridization)

Expression Phenotype
increased amount ventx1.2 expression in ventral marginal zone decreased amount trim33 expression in ectoderm increased amount pou5f3.3 expression in animal pole

Fig. 4a,b, e, f, k, l, m, n

Sempou E et al. (2022)

Xtr Wt + kcnh6 CRISPR (400pg)

NF10 (in situ hybridization)

Anatomical Phenotype
abnormal gastrulation abnormal mesoderm

Expression Phenotype
increased amount ventx1.2 expression in embryo absent myod1 expression in dorso-lateral marginal zone decreased amount pou5f3.3 expression in embryo decreased amount sox2 expression in dorsal side decreased amount trim33 expression in animal cap decreased amount trim33 expression in endomesoderm increased amount pou5f3.1 expression in embryo

Fig. 5 a_c2,d, b_c2,e, c_c2,f

Sempou E et al. (2022)

Xtr Wt + kcnh6 CRISPR (400pg)

NF10 (in situ hybridization)

Expression Phenotype
increased amount ventx1.2 expression in ventral marginal zone decreased amount trim33 expression in ectoderm increased amount pou5f3.3 expression in animal pole

Suppl. Fig. 6 b r1_c3, r2_c3

Sempou E et al. (2022)

Xtr Wt + kcnh6 CRISPR (400pg)

NF12 (in situ hybridization)

Expression Phenotype
increased amount ventx1.2 expression in animal pole increased amount ventx1.2 expression in ventral marginal zone increased amount pou5f3.1 expression in embryo

Fig.4.I

Gur M et al. (2022)

Xla Wt + bmp4

NF10.25 (RT-PCR)

Anatomical Phenotype
partially ventralized embryo

Expression Phenotype
increased amount ventx1.2.L expression in embryo decreased amount admp.L expression in the embryo decreased amount aldh1a3.L expression in embryo decreased amount gsc.L expression in the embryo increased amount szl.L expression in embryo

Fig.4.I

Gur M et al. (2022)

Xla Wt + bmp4

NF11 (RT-PCR)

Anatomical Phenotype
partially ventralized embryo

Expression Phenotype
increased amount ventx1.2.L expression in embryo decreased amount admp.L expression in the embryo decreased amount aldh1a2.L expression in the embryo decreased amount aldh1a3.L expression in embryo decreased amount gsc.L expression in the embryo increased amount szl.L expression in embryo

Fig.4.I

Gur M et al. (2022)

Xla Wt + bmp4

NF13 (RT-PCR)

Anatomical Phenotype
partially ventralized embryo

Expression Phenotype
decreased amount ventx1.2.L expression in embryo decreased amount admp.L expression in the embryo decreased amount aldh1a2.L expression in the embryo decreased amount aldh1a3.L expression in embryo decreased amount gsc.L expression in the embryo

Fig.4.H

Gur M et al. (2022)

Xla Wt + wnt8a

NF10.25 (RT-PCR)

Anatomical Phenotype
partially dorsalized embryo

Expression Phenotype
decreased amount ventx1.2.L expression in embryo decreased amount szl.L expression in the embryo increased amount admp.L expression in the embryo increased amount aldh1a3.L expression in the embryo increased amount gsc.L expression in the embryo increased amount aldh1a2.L expression in embryo