XB-PHENO-23044
Xtr Wt + osr1 MO + osr2 MO + RA, Rankin SA et al. (2012)
| Experiment + Assay: |
Xtr Wt + osr1 MO + osr2 MO + RA +
NF35/36 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
osr1 MO1
|
osr1
|
NF stage 4 (8-cell)
|
blastomere
|
|
morpholino injection
|
osr2 MO1
|
osr2
|
NF stage 4 (8-cell)
|
blastomere
|
|
chemical
|
Retinoic acid
|
|
NF stage 25
|
whole organism
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 35 and 36
|
|
|
|
|
Expression Phenotypes (4):
|
|
increased amount
aldh1a2
expression in lateral plate mesoderm
|
|
mislocalised
aldh1a2
expression in pharyngeal region
|
|
decreased amount
nkx2-1
expression in lung bud
|
|
decreased amount
wnt2b
expression in lateral plate mesoderm
|
|
