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Xla Wt + VP16-ventx2.2-GR + DEX, McLin VA et al. (2007)

Experiment + Assay: Xla Wt + VP16-ventx2.2-GR + DEX + NF35/36-37 and 38 (in situ hybridization)
Background: Xla.Outbred
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(VP16-ventx2.2-GR) ventx2.2.L
NF stage 6 (32-cell) blastomere D2.1.1
chemical dexamethasone
NF stage 7 embryo
Assay Type Assay Stages Antibody
in situ hybridization NF stage 35 and 36 to NF stage 37 and 38  
Expression Phenotypes (3):
decreased amount nr1h5.L expression in liver
decreased amount pdx1.L expression in dorsal pancreatic bud
decreased amount pdx1.L expression in ventral pancreatic bud
Source: Fig.S.2.C
Article: XB-ART-35992, PubMed