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Summary Attributions
XB-TRANSGENE-22251778
Transgene Name Tg(six1-GR)
Description To generate a hormone-inducible Six1expression vector (pCS2+-GR-myc-Six1), the full open-reading frame of Xenopus Six1 was generated by PCR with primers containing XhoI sites and the pDH105-Six1 plasmid (Brugmann et al., 2004). The Six1fragment was inserted into the XhoI site downstream of GR-myc in pCS2+-GR-myc. Both plasmids were confirmed by sequencing and used as templates to generate mRNAs (GR–Six1).
Inducibility None

Components

Type
Sequence No sequence available
Organism

Type
Sequence No sequence available
Organism



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